Table 3.

List of primers used for qPCR

Universal antisense	5′-GAA CAG TAT GTG TCA CAG ACG TAC-3′
Let-7a	5′-GCGG TGAGGTAGTAGGTTGTAT-3′
Let-7d	5′-GCGG AGAGGTAGTAGGTTGCATA-3′
miR-10a	5′-GCATG TAC CCT GTA GAT CCG A-3′
miR-15a	5′-GCGG TAGCAGCACATAATGGTT-3′
miR-15b	5′-CATG CAT AGC AGC ACA TCA TG -3′
miR-19b	5′-CATG TGT GCA AAT CCA TGC A -3′
miR-20a	5′-GCGG TAAAGTGCTTATAGTGCAG-3′
miR-21	5′-GCATGCA TAG CTT ATC AGA CTG -3′
miR-25	5′ -TCA TTG CAC TTG TCT CGG T -3´
miR-100	5′-GCATT AAC CCG TAG ATC CGA-3′
miR-103	5′-CGG AGCAGCATTGTACAGG-3′
miR-125b	5′-GCAT TCC CTG AGA CCC TAA C-3′
miR-155	5′-GCATGCA TTA ATG CTA ATC GTG A -3′
miR-191	5′-GCGG CGG AAT CCC AAA AGC AG-3′
miR-222	5′-GCATG CTCAGTAGCCAGTGTAG-3′

The part of capital letters represents the miRNA-specific primer, the uncapitalized part represents the melting temperature overhang