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. 2022 Jul 20;14:38. doi: 10.1038/s41368-022-00188-y

Fig. 3.

Fig. 3

h-DPSCs treated with a range of BMP7 concentrations at 2, 10, 50, and 250 ng·mL−1. a CCK8 assay for the evaluation of h-DPSCs proliferation. b, c The wound-healing assay and transwell assay for evaluation of h-DPSCs migration ability. d, e Quantification of the relative invasion and migration cell number in b and c. f Alizarin red staining of h-DPSCs induced by osteogenic medium contained with or without BMP7 (2, 10, 50, and 250 ng·mL−1) for 21 days. g The expression of osteo/odontogenic markers of h-DPSCs after 3 days of culture. *P < 0.05, #P < 0.01 vs Control. NS: no significance. Scale bar: b 200 μm, c 100 μm, f 500 μm