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. 2022 Jul 20;13:4188. doi: 10.1038/s41467-022-31904-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Mini-WARS overexpression decreases histamine-elicited endothelial permeability. Quantification of 70 kDa Dextran-FITC transendothelial flux over time through a confluent Ctrl (empty lentivirus) or mini-WARS-transduced EC monolayer after histamine stimulation. Data are the mean ± SEM of three independent experiments. Statistical analysis: two-way ANOVA with Bonferroni correction; *P value = 0.0126, **P value = 0.0020, 0.0016. Western blot analysis shows mini-WARS overexpression in ECs. b Mini-WARS silencing impairs histamine-elicited endothelial permeability. Quantification of 70 kDa Dextran-FITC transendothelial flux over time through a confluent control (siCtrl) or WARS (siWARS) silenced ECs rescued for empty vector (Ctrl/siCtrl and Ctrl/siWARS) or for mini-WARS (mini-WARS/siWARS) ECs monolayer after histamine stimulation. Data are the mean ± SEM of three independent experiments. Statistical analysis: two-way ANOVA with Bonferroni correction; **P value ≤ 0.01. Western blot analysis shows WARS silencing and mini-WARS overexpression. c Mini-WARS overexpression decreases histamine-elicited endothelial permeability via NRP1. Quantification of 70 kDa Dextran-FITC transendothelial flux over time through a confluent control (siCtrl, left) or NRP1 silenced (siNRP1, right) and transduced with empty lentivirus (Ctrl) or mini-WARS EC monolayer after histamine stimulation. Data are the mean ± SEM of three independent experiments. Statistical analysis: two-way ANOVA with Bonferroni correction; ns = P value ≥ 0.05, **P value = 0.0047, 0.0070, 0.0010. Western blot analysis shows NRP1 silencing and mini-WARS overexpression in ECs. Source data are provided as a Source Data file.