Fig. 1. The 2-step cell-crush method for in vivo RNA-Seq analysis of S. aureus in mouse liver.

C57BL/6J mice were infected with S. aureus Newman strain injected through the tail vein, and organs were harvested at 6, 24, and 48 h.p.i. Tissue and bacterial cells were separately crushed and lysed in lysis buffer using beads of 2 different sizes.