Table 3.

Gene expression change in the S. aureus 2-component system and virulence-related transcription factors in the host liver compared with culture medium.

	Gene	Function	6 h.p.i.		24 h.p.i.		48 h.p.i.
			Fold	FDR p-value	Fold	FDR p-value	Fold	FDR p-value
Two-component factors37	agrC	Quorum sensing control of adhesion and virulence factors	1	1	−1.2	0.765	−2.3	0.013
	agrA		1.2	0.696	1.2	0.672	−1.9	0.026
	saeS	Virulence factors regulation (toxins, enzymes)	1.1	0.743	1.1	0.848	−4.7	<0.001
	saeR		1	0.996	−1.1	0.918	−4.2	<0.001
	vraR	Cell wall -affecting antibiotic resistance, cell wall biosynthesis	2.3	<0.001	1.7	0.024	−2	0.009
	vraS		2.1	0.001	1.3	0.279	−2.6	0.005
	graX	AMP resistance, growth at low pH	1.9	0.007	1.1	0.951	1	0.996
	graR		2.7	<0.001	−1	0.998	−1.3	0.545
	graS		1.9	0.007	1.3	0.464	−1.2	0.694
	braS	Antimicrobial peptide resistance	1.2	0.588	1.3	0.365	−1.2	0.581
	braR		−1.4	0.468	−1.4	0.54	1.1	0.885
	arlS	Pathogenesis mechanisms: autolysis, adhesion, biofilm	1.4	0.199	−1.2	0.576	−2	0.016
	arlR		−1.5	0.18	−2.1	0.022	−1.9	0.023
	WalR	cell wall maintenance, cell viability	−1.2	0.612	−1.2	0.583	−1.3	0.613
	WalK		1	1	−1.2	0.738	−1.4	0.166
	hptS	intercellular survival, uptake of hexose phosphate	−2.2	0.115	1.1	0.922	1.6	0.21
	hptR		−3.3	0.012	−2.8	0.029	1.9	0.04
	tcs7R	Uncharacterized function	4.9	<0.001	3	0.001	1.9	0.085
	tcs7S		3.2	<0.001	2.7	0.005	1.4	0.371
	srrB	Anaerobic respiration, metabolism, growth at low temperature	−1.8	0.027	−1.5	0.141	1.3	0.443
	srrA		−1.4	0.303	−1.4	0.316	2.1	0.03
	phoR	Phosphate uptake and homeostasis	5.3	<0.001	3.4	<0.001	1.2	0.486
	phoP		1.2	0.555	−1.1	0.871	1.6	0.252
	airR	Oxidative stress response	−1.3	0.634	1.6	0.272	−1.8	0.089
	airS		1.1	0.802	1	0.998	−1.1	3831
	kdpD	Potassium homeostasis regulation	1.4	0.545	3.5	<0.0001	3.7	<0.001
	kdpE		2.1	0.123	3.1	0.005	4.4	<0.001
	hssR	Heme metabolism regulation	−1.7	0.317	−5.3	0.018	1.7	0.228
	hssS		1.8	0.058	−1.1	0.888	−1.1	0.826
	nreC	Response to low oxygen, nitrate reduction	1.3	0.337	1.2	0.576	1.2	0.56
	nreB		1.1	0.872	1.2	0.635	1.6	0.177
Transcription factor27	sarA	Positive to agr expression, induction of exoproteins and repression of spa	−1.6	0.051	1.7	0.118	−5.4	<0.001
	sarH1	Repressor of agr, sarA	−13.6	<0.001	−8.7	<0.001	−4.3	<0.001
	sarU	Positive to agr expression,	−5.2	0.925	18.6	0.001	4.3	<0.001
	sarR	Negative to agr, sarA	1.1	0.792	1.1	0.801	−5.2	<0.001
	sarT	Negative to agr, hla, sarU expression	−9.4	0.868	6.9	0.039	5.3	<0.001
	sarX	Negative to agr	−8.6	0.001	−1.2	0.778	−1.7	0.255
	sarZ	Positive to agr38	−1.7	0.041	−1.5	0.136	−2.6	0.009
	CcpA	Positive to agr	1.8	0.015	−1.1	0.797	−2.3	0.011
	codY	Negative to agr	1.8	0.001	1.4	0.257	−1.1	0.783
	mgrA	Cytoplasmic regulator; induction of efflux pumps and capsule expression; repress of surface proteins, Positive to agr, sarZ, sarX	−1.9	0.017	1.1	0.966	−4.6	<0.001
	rsr	Repressor of agr39	4.9	<0.001	2.3	0.003	−1.9	0.092
	sigB	Stress response	−2.4	<0.001	−2.6	<0.001	−2.2	<0.001
	yjbH	Virulence factor regulator28	5.6	<0.001	2.4	<0.001	−1.4	0.139