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. 2022 Jul 20;5:720. doi: 10.1038/s42003-022-03683-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Cartoon of the ciliary segments and representative single-molecule kymographs of OCR-2::EGFP. Scale bars, vertical: 1 μm, horizontal: 1 s. b Single-molecule kymographs showing TZ crossings of Kinesin-II (KAP-1::EGFP) and OCR-2::EGFP kap-1(ok676). Color bars indicate ciliary segment as in (A). Scale bars, vertical: 1 μm; horizontal: 1 s. c Anterograde (A) and retrograde (R) velocity of KAP-1::EGFP (A: 63 trajectories; R: 273 trajectories), OCR-2::EGFP (A: 51 trajectories; R: 297 trajectories) and OCR-2::EGFP kap-1(ok676) (A: 19 trajectories; R 141 trajectories). Velocities were extracted using KymographDirect from kymographs optimized for the TZ (a different data set than used in the rest of the manuscript). Data are represented as boxplot. Indicated are median (block horizontal line), 25th–75th percentile (height of the colored boxes), 5th–95th percentile. The widths of the boxes represent the sample size.