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. 2022 Jul 20;5:720. doi: 10.1038/s42003-022-03683-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Example single-particle trajectories from the kymographs of Fig. 2a. b Mean squared displacement (MSD) of annotated trajectories in a, demonstrating active transport (*), normal diffusion (+), and subdiffusion (#). c Log–log plot of the first six time points. d Cartoon explaining the single-molecule data analysis with a sliding window. e Histogram and corresponding violin plot of normalized probability of α_// for all 7237 data points obtained from 23 nematodes. Colored lines represent two underlying Gaussian distributions.