Figure 5.

Adoptive transfer of TGF-β + GM-CSF–induced MDSCs could effectively prolong the survival of allo-skin. The recipient female B6 mice were injected intravenously (i.v.) with induced MDSCs (5 × 10⁶) on day 1 before operation and day 7 after operation. The mice in the control group were injected i.v. with vehicle PBS. In addition, TGF-β + GM-CSF–induced MDSCs and Arg-1 inhibitor, nor-NOHA, was administered to another group. (A) The photographs of the graft rejection have been shown in the four different groups. (B) Representative HE staining of skin grafts on day 21 has been shown (400×). (C) Quantification of H&E staining corresponding to the four groups on day 21. (D) The survival time of the graft after transplantation have been summarized (n = 6). P values were determined by log-rank tests. *P < 0.05, **P < 0.01, and ***P < 0.001 compared between the different groups. (E) The levels of the donor-specific antibodies (DSAs), IgG1, IgG2a, and IgG2b were detected by FCM analysis. The data have been shown as mean ± SEM (n = 3) and were analyzed by one-way ANOVA analysis. *P < 0.05, **P < 0.01, and ***P < 0.001 compared between the different groups.