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. 2022 Jul 7;13:919674. doi: 10.3389/fimmu.2022.919674

Figure 6.

Figure 6

Adoptive transfer of TGF-β + GM-CSF–induced MDSCs could significantly promote the expansion of MDSCs and Tregs in the transplant recipients. Allo-skin mice were divided into four different groups and then treated with PBS, GM-CSF–induced MDSCs, TGF-β + GM-CSF–induced MDSCs, and TGF-β + GM-CSF–induced MDSCs with Arg-1 inhibitor, respectively. Thereafter, allografts, peripheral blood, and spleen of transplant recipients were obtained on day 21 after operation for FCM assay. Typical examples of FCM analysis and proportion of CD11b+Gr-1+ MDSCs in allografts (A), peripheral blood (B), and spleen (C) of the transplanted mice. Typical examples of flow cytometry analysis and percentages of CD4+Foxp3+ Tregs in allografts (D), peripheral blood (E), and spleen (F) of the transplant mice. The data have been shown as mean ± SEM (n = 3) and were analyzed by one-way ANOVA analysis. *P < 0.05, **P < 0.01, and ***P < 0.001 compared between the different groups.