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Sensitivity of the duplex iiPCR. .
| Copy number/reaction | Genotype I (550 nM) | Genotype II (520 nM) | NARL-recommend Real-time PCR (Ct) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 2000 | + | + | + | + | + | + | + (31.12) | + (30.55) | + (31.8) |
| 200 | + | + | + | + | + | + | + (34.77) | + (34.25 | + (34.51) |
| 20 | + | + | + | + | + | + | + (37.16) | + (37.58) | + (36.99) |
| 2 | – | – | – | – | – | – | - (N/A) | - N/A) | - (N/A) |
Each dilution of plasmids was tested for 3 times. The cut-off Ct value for NARL-recommend real-time PCR was set as 38 according to the manual instruction.
