Table 4.

Application of the duplex iiPCR on clinical ASFV positive DNA samples.

Sample No.	DNA extracts from positive samples	NARL-recommend real-time PCR (Ct value)	Duplex iiPCR
			Genotype I (550nM)	Genotype II (520nM)
1	Lymph node	32.20	–	+
2	Lymph node	28.54	–	+
3	Lymph node	33.01	–	+
4	Lymph node	18.66	–	+
5	Lymph node	19.84	–	+
6	Liver	19.66	–	+
7	Liver	17.13	–	+
8	Lung	18.36	–	+
9	Lung	22.14	–	+
10	Lung	15.45	–	+
11	Spleen	23.13	–	+
12	Spleen	21.93	–	+
13	Spleen	22.47	–	+
14	Spleen	16.89	–	+
15	Spleen	16.32	–	+
16	Spleen	14.95	–	+
17	Spleen	16.92	–	+
18	Serum	22.31	–	+
19	Serum	15.71	–	+
20	Serum	20.14	–	+
21	Serum	22.47	–	+
22	Serum	17.26	–	+
23	Serum	18.55	–	+
24	Serum	35.98	–	+
25	Serum	24.29	–	+
26	Serum	25.44	–	+
27	Serum	25.67	–	+
28	Serum	18.05	–	+
29	Serum	16.31	–	+
30	Serum	15.77	–	+
31	Serum	19.38	–	+
32	Serum	17.68	–	+
33	Serum	18.17	–	+
34	Serum	23.42	–	+
35	Serum	19.15	–	+
36	Serum	20.54	–	+
37	Serum	22.85	–	+
38	Serum	25.90	–	+
39	Serum	13.79	–	+
40	Serum	32.66	–	+
41	Serum	17.84	–	+
42	Serum	30.79	–	+
43	Environmental swab	33.12	–	+
44	Environmental swab	26.89	–	+
45	Environmental swab	26.24	–	+
46	Environmental swab	31.38	–	+
47	Environmental swab	29.74	–	+
48	Environmental swab	32.69	–	+
49	Environmental swab	35.74	–	+
50	Environmental swab	33.55	–	+
51	Environmental swab	36.42	–	+
52	Environmental swab	36.65	–	+
53	Environmental swab	33.10	–	+
54	Environmental swab	32.67	–	+
55	Environmental swab	34.89	–	+
56	Environmental swab	33.92	–	+
57	Environmental swab	25.94	–	+
58	Environmental swab	24.01	–	+
/	E70 DNA	18.56	+	–
/	Ba71V DNA	21.80	+	–
/	E75 DNA	24.91	+	–
/	Pos Ctrl	25.44	+	+
/	Neg Ctrl	N/A	–	–