Table 5.

A Direct application of serum samples without DNA extraction and tested on the duplex iiPCR.

Sample No.	Duplex iiPCR
	Genotype I (550nM)	Genotype II (520nM)
1	–	+
2	–	+
3	–	+
4	–	+
5	–	+
6	–	+
7	–	+
8	–	+
9	–	+
10	–	+
11	–	+
12	–	+
13	–	+
14	–	+
15	–	+
16	–	+
17	–	+
18	–	+
19	–	+
20	–	+
21	–	+
22	–	+
23	–	+
24	–	+
25	–	+
26	–	–
27	–	–
28	–	–
29	–	–
30	–	–
31	–	–
32	–	–
33	–	–
34	–	–
35	–	–
Pos Ctrl	+	+
Neg Ctrl	–	–