Table 6.
Sensitivity comparison of the duplex iiPCR using unextracted or extracted ASFV genotype II positive serum sample.
| Dilution | Unextracted ASFV positive serum | Extracted DNA | ||||
|---|---|---|---|---|---|---|
| No.42 | No.3 | No.24 | No.42 | No.3 | No.24 | |
| Undiluted | +/- | +/- | +/- | +/- | +/- | +/- |
| 10-1 | +/- | +/- | -/- | +/- | +/- | -/- |
| 10-2 | +/- | -/- | -/- | +/- | -/- | -/- |
| 10-3 | -/- | -/- | -/- | -/- | -/- | -/- |
Three serum samples with different Ct values detected by real-time PCR in Table4 were used. The Ct value of each sample was shown under the number of serum samples. The results of each sample were shown by reading at 520/550 nM on the device.