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. 2022 Jul 7;13:942180. doi: 10.3389/fphar.2022.942180

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Copyright © 2022 Guo, Liu, Wang, Zheng, Huang, Kong, Ding, Lv, Wang, Jiang, Jiang and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Luteolin affected the hemolytic activity of SLO and culture-supernatant. (A,B) RBCs were treated with 0, 0.1, 0.2, and 0.4 μg/ml of SLO or 0%, 7.5%, 10%, and 12.5% of GAS culture-supernatant for 30 min at 37°C. (C) Effect of luteolin on RBC lysis induced by SLO. (D) Effect of luteolin on the hemolytic activity of GAS culture-supernatant. The OD at 540 nm of each sample was obtained. Results were represented as the percentage of lysed RBCs (assuming as 100% the positive control) derived from three independent experiments ± SD (One-way ANOVA, ***p < 0.001 compared with cells treated with 0.1 μg/ml of SLO in the absence of luteolin).