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. 2022 Jul 7;16:910662. doi: 10.3389/fncel.2022.910662

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Copyright © 2022 Guerra Leal, Barros-Barbosa, Ferreirinha, Chaves, Rangel, Santos, Carvalho, Martins-Ferreira, Samões, Freitas, Lopes, Ramalheira, Lobo, Martins da Silva, Costa and Correia-de-Sá.

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Representative confocal micrographs of different sub-regions of the human hippocampus showing that the P2X7R immunoreactivity (green) is higher in the hippocampus of MTLE-HS patients than of non-epileptic cadaveric controls. A negative control resulting from incubation of the DG/CA4 hippocampal region of an MTLE-HS patient with the anti-rabbit secondary antibody without previous addition of the rabbit anti-P2X7R primary antibody (#APR-004) and differential interference contrast (DIC) images are shown for comparison; three confocal micrographs were obtained per individual; three individuals from each group (control and MTLE-HS) were analyzed showing similar results; scale bars = 300 μm.