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. 2022 Jul 7;10:879853. doi: 10.3389/fped.2022.879853

Figure 1.

Figure 1

Study flowchart. HM, human milk; DT-HoP, dry-tempering Holder pasteurization; WB-HoP, water-bath pasteurization; HTST, high-temperature short-time treatment; ALP, alkaline phosphatase; sIgA, secretory immunoglobulin A; LF, lactoferrin. *Preparation of milk whey: From samples 10 mL aliquots were centrifuged at 600 g for 10 min, the resulting aqueous layer was again centrifuged at 600 g for 10 min and the resulting whey supernatant was centrifuged at 2,500 g for 10 min and the resultant supernatant was then filtrated with a 0.45 μm with syringe filter.