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PCR primers.
| Purpose | Name | Sequence (5′…) | Size of amplicon |
|---|---|---|---|
| Detection of BFo1 | BFo1PEG1885sF | GATCAACCCGCGCTGTTATC | 200 bp |
| BFo1PEG1885sR | CTGGCGGATATCCTCAACCA | ||
| Detection of BFo2 | BFo1PEG1526MF | CTGGAGCCCTGAAGTGAGAA | 350 bp |
| BFo1PEG1526MR | CTGCTCACTCTCTGGGTTGA | ||
| Amplification of GFP | GFP-RVF | AGTACTGATATCCACTAGTGAGCTCGTTGCGC | 805 bp |
| GFP-RVR | GTCAATGATATCCAACAGGAGTCCAAGCTCAGC |
