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. 2022 Jul 7;16:938874. doi: 10.3389/fnins.2022.938874

FIGURE 3.

FIGURE 3

Nobiletin (NOB) attenuated systemic cytokine levels and prefrontal cortical inflammation as assessed by enzyme-linked immunosorbent assay (ELISA). Vehicle or NOB at doses of 1, 10, 30, and 50 mg/kg body weight were intraperitoneally injected, respectively, once per day for 7 consecutive days before surgery. Mice underwent exploratory laparotomy under sevoflurane anesthesia the next day after NOB pretreatment. Blood (A,B), prefrontal cortex (C,D,E) and hippocampus (F,G,H) were harvested 24h after surgery to test proinflammatroy cytokine by ELISA. C0: control group without surgery; S0: surgery group with vehicle pretreatment S1,S10,S30,S50: surgery group with NOB 1,10,30, 50mg/kg pretreatment. The value of the C0 group was set to 1. # P < 0.05, ## P < 0.01, ### P < 0.001. n = 6, compared with the C0 group. *P < 0.05, **P < 0.01, ***P < 0.001, n = 6, compared with the S0 group. Results were presented as means ± S.E.M.