Figure 3:

Sensitivity of Txnrd1-deficient β-cells to oxidative damage. (A) Phosphorylation of the histone variant H2AX (γH2AX, marker of DNA damage response activation) as assessed by western blot using protein lysates from Txnrd1^fl/fl, Ins1^+/+ (fl/fl) islets with or without 5 μm AFN or from Txnrd1^fl/fl, Ins1^Cre/+ (Cre⁺) islets following 30 min exposure to hydrogen peroxide delivered continuously by 15 mU/mL glucose oxidase or to 60 min exposure to peroxynitrite, delivered by the combination of DPTA/NO and menadione. α-Tubulin levels were determined to assess protein loading. A representative blot is shown. (B) Quantification of bands shown in (A). Error bars represent SEM. Results are the average of 2–3 independent experiments.