Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 4;3(4):zqac034. doi: 10.1093/function/zqac034

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of American Physiological Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 5. — Nrf2 is stabilized in Txnrd1-deficient β-cells. (A) Predicted upstream regulators based on significantly changed genes in Txnrd1 knockout GFP⁺ cells compared to control GFP⁺ cells. Analysis was performed using IPA. (B) Expression of Nfe2l2 in Txnrd1^+/+, Ins1^Cre/+ (+/+; Cre⁺) or Txnrd1^fl/fl, Ins1^Cre/+ (fl/fl; Cre⁺) β-cells as determined by RNA-sequencing. (C) Nrf2 protein levels as assessed by western blot in Txnrd1^fl/fl, Ins1^+/+ (fl/fl) and Txnrd1^fl/fl, Ins1^Cre/+ (Cre⁺) islet lysates. α-Tubulin levels were determined to assess protein loading. Band quantification is shown above a representative blot. (D) Heat map showing Log₂ (RPKM) of selected genes in four Txnrd1^+/+, Ins1^Cre/+ (+/+; Cre⁺) β-cell samples, and three Txnrd1^fl/fl, Ins1^Cre/+ (fl/fl; Cre⁺) β-cell samples as determined by RNA-sequencing. All genes shown are significantly different between the genotypes. (E) Expression of Hmox1, Gpx2, and Srxn1 in Txnrd1^fl/fl, Ins1^+/+ (fl/fl), and Txnrd1^fl/fl, Ins1^Cre/+ (Cre⁺) islets as determined by qPCR. (F) HO-1 protein levels as assessed by western blot in Txnrd1^fl/fl, Ins1^+/+ (fl/fl) and Txnrd1^fl/fl, Ins1^Cre/+ (Cre⁺) islet lysates. α-Tubulin levels were determined to assess protein loading. Band quantification is shown to the right of a representative blot. Results are the average of 3–6 independent experiments with statistical significance indicated. *P <0.05 (vs. fl/fl).