Skip to main content
. 2022 Jul 7;13:952235. doi: 10.3389/fimmu.2022.952235

Figure 1.

Figure 1

Analysis of IgG subclasses of anti-THSD7A autoAbs. (A) Standard curves show the concentration dependence for the binding of recombinant human anti-TNFα IgG1 (blue), IgG2 (orange), IgG3 (green) and IgG4 (red) to wells coated with TNFα antigen. (B) Sera from 16 patients with THSD7A-associated MN were analyzed by ELISA for IgG autoAb subclasses binding to immobilized THSD7A. The serum concentrations of anti-THSD7A IgG1 (blue), IgG2 (orange), IgG3 (green) and IgG4 (red) in each MN serum were interpolated from standard curves using recombinant human IgG1-IgG4 mAbs anti-TNFα. Sera were designated MN-1 to MN-16 in decreasing order of their levels of anti-THSD7A IgG, calculated as the sum of the concentrations of all four IgG subclasses. (C) The correlation between serum levels of anti-THSD7A IgG measured by ELISA and the anti-THSD7A titers measured by indirect immunofluorescence test (IIFT) was significant (Pearson r=0.849, p=0.0001). (D) The relative proportion of IgG subclasses in MN sera was calculated by dividing the serum concentration of each subclass to sum of concentrations for all four IgG subclasses of autoAbs. The error bars show the median and the interquartile range.