Table 2.
Calibration equationsa, correlation coefficients, limits of detection (LOD), limits of quantification (LOQ), and recovery rates for FA, FAX, and FAXG using HSQC-NMR detection.
| Range tested (μM) | Linear calibration equation | Correlation coefficients (R2) | LOD (μM) | LOQ (μM) | Recovery rate (%) | |
|---|---|---|---|---|---|---|
| FA | 470–2,400 | y = 99,366x – 0.0133 | 0.9829 | 307 | 930 | 103 |
| FAX | 120–440 | y = 102,034x – 0.0044 | 0.8578 | 108 | 327 | 75 |
| FAXG | 120–350 | y = 110,243x – 0.0076 | 0.7165 | 153 | 463 | 83 |
FA, 5-O-trans-feruloyl-l-arabinofuranose; FAX, β-d-xylopyranosyl-(1 → 2)-5-O-trans-feruloyl-l-arabinofuranose; FAXG, α-l-galactopyranosyl-(1 → 2)-β-d-xylopyranosyl-(1 → 2)-5-O-trans-feruloyl-l-arabinofuranose; HSQC, heteronuclear single quantum coherence spectroscopy; NMR, nuclear magnetic resonance.
a
Calibration equations and correlation coefficients calculated from triplicate calibrations. Recovery rates were determined in duplicate.