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. 2022 Jul 7;15:859349. doi: 10.3389/fnmol.2022.859349

FIGURE 2.

FIGURE 2

N-Fbs facilitates Schwann cells migration and proliferation in vitro. (A) Phase-contrast micrographs of crystal violet-labeled migrated SCs co-cultured with blank control, C-Fbs, and N-Fbs in transwell for 6 h, 12 h, and 24 h. Scale bar, 50 μm. (B) Migrating SCs quantification (three independent experiments). Statistical significance is indicated as *p < 0.05 N-Fb-12h vs. Control-12h, **p < 0.01 C-Fb-24h vs. Control-24h, ***p < 0.001 N-Fb-24h vs. C-Fb-24h, ****p < 0.0001 N-Fb-24h vs. Control-24h (two-way analysis of variance followed by Tukey’s post hoc test). (C) EdU+ SCs (red) were labeled after 24-h co-culture with N-Fbs and C-Fbs, separately. Scale bar, 75 μm. (D) Quantification of the proportion of EdU+ SCs (three independent experiments). Statistical significance is indicated as **p < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test).