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. 2022 Jul 6;15:901466. doi: 10.3389/fnmol.2022.901466

Figure 5.

Figure 5

Attenuated response to static touch and light mechanical pain in Meis1cko mice. (A) The rotarod assay. Control and Meis1cko mice showed identical latencies to fall off the rotarod (n = 5 control mice, n = 4 Meis1cko mice; p > 0.05). (B) Acute mechanical pain assessed using the pinprick test. Control and Meis1cko mice showed no significant difference (n = 8 mice per group; p > 0.05). (C) Von Frey assay. Meis1cko mice showed a significantly higher withdrawal threshold than control littermates, and the threshold was increased from 0.43 ± 0.044 g in control mice to 0.58 ± 0.046 g in Meis1cko mice (n = 12 mice per group; p = 0.0187). (D) The latency to remove the sticky tape on the hindpaw was 72 ± 11 s for control mice and 101 ± 10 s for Meis1cko mice (n = 12 mice per group; p = 0.0445). (E) Number of scratching bouts to remove adhesive tape on nape hairy skin (61 ± 8 for control mice vs. 46 ± 8 for Meis1cko mice, n = 10 mice per group; p = 0.187). (F) Percent response to light stroking with a cotton swab (n = 12 mice per group; p > 0.05). (G) Cooling sensation tested by hindpaw exposure to acetone (n = 9 control mice, n = 8 Meis1cko mice; p > 0.05). (H) The latency of forepaw flinching or hindpaw licking on a 0°C cold plate (n = 10 control mice, n = 6 Meis1cko mice; p > 0.05). (I) The time that mice spent licking and flinching after the intraplantar capsaicin injection (n = 5 mice per group; p > 0.05). (J) Hot plate assay. No statistically significant difference was observed between control and Meis1cko mice at 50, 52, and 55°C (n = 12 mice per group, p > 0.05). (K) Meis1cko mice showed a normal response to CFA-induced inflammatory pain (two-way repeated ANOVA, n = 7 mice per group; p > 0.05). (L) Meis1cko mice showed a normal response to SNI-induced neuropathic pain (two-way repeated ANOVA, n = 6 control mice, n = 8 Meis1cko mice; p > 0.05). Error bars represent SEM. (A–J) Significant differences were determined using unpaired Student's t-test: *p < 0.05 ns, Not significant.