FIG. 1.

Restriction endonuclease maps of the P. stutzeri OX1 chromosomal DNA fragment cloned in pFB3401 and its derivatives (3). pFB3401 allowed the host cells to convert toluene, o-xylene, and their phenolic derivatives into the corresponding ring fission products. The ToMO-encoding operon and the regulatory gene (touR) controlling its expression are indicated (1, 4). The point of the arrows indicates the direction of transcription. The ability (+) or inability (−) of the plasmids to direct the conversion of o-cresol into ring fission products in the absence (−) or in the presence (+) of touR, cloned in pRZ7085 and provided in trans, is indicated to the right of the restriction maps. +/−, mildly positive reaction after prolonged exposure. Abbreviations: A, ApaI; B, BamHI; E, EcoRI; H, HindIII; N.A., not applicable; P, PstI; S, SacI; X, XhoI; Xb, XbaI. Only relevant restriction sites are indicated.