FIG. 2.

Restriction endonuclease maps of DNA fragments subcloned from pFB1021 (pJ and pBZ series) and from pFB3411 (pVS series). Only the plasmids containing the minimal region coding for PH, C2,3O, HMSH, and HMSD are reported. The black arrowheads indicate the position of the Plac of the vector. To the right of the restriction maps, the enzymatic activities expressed from the indicated plasmids in uninduced (−) or IPTG-induced (+) E. coli JM109 cells are reported. At the bottom, the map obtained by subcloning, Southern, and sequence analyses (see text for details) displaying the gene arrangement and the putative promoter is shown. The presence of the gene indicated in parentheses can be hypothesized but has not been investigated. Abbreviations: B, BamHI; E, EcoRI; H, HindIII; M, MluI; ND, not detectable; P, PstI; S, SacI; Sm, SmaI; X, XhoI; Xb, XbaI. Only relevant restriction sites are indicated.