TABLE 2.
Substrate range and reaction product identification for the P. stutzeri OX1 PH from IPTG-induced E. coli JM109(pJSX148) cells
| Substrate | PH sp act (nmol min−1 mg of protein−1) | HPLC retention time of product (min) | Product identified | HPLC retention time for standard of predicted product (min) | Cleaved by C2,3O |
|---|---|---|---|---|---|
| Phenol | 8.2 ± 0.5 | 3.55 | Catechol | 3.51 | + |
| o-Cresol | 10.8 ± 1.0 | 3.44 | 3-Methylcatechol | 3.40 | + |
| m-Cresol | 8.7 ± 0.7 | 3.35 | 3-Methylcatechol | 3.40 | + |
| p-Cresol | NAa | 3.16 | 4-Methylcatechol | 3.13 | + |
| 2,3-DMP | 12.7 ± 0.5 | 4.25 | 3,4-Dimethylcatechol | 4.05 | + |
| 3,4-DMP | NA | 4.17 | 3,4-Dimethylcatechol | 4.05 | + |
| 2,4-DMP | NA | 4.10 | 3,5-Dimethylcatecholb | NAvc | − |
| 3,5-DMP | 11.4 ± 1.0 | 4.07 | Not analyzed | NAv | − |
| 2,5-DMP | 14.4 ± 2.8 | 4.21 | 3,6-Dimethylcatecholb | NAv | − |
a
NA, Not applicable.
b
Identified by NMR analysis.
c
NAv, not available.