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. 2022 Jul 5;7(28):24344–24352. doi: 10.1021/acsomega.2c01700

Figure 2.

Figure 2

Cocrystal structure of FOXO3apS253 peptide and 14-3-3εΔC. (A) Ribbon representation of the 14-3-3ε dimer with each monomer (green cartoon) binding a FOXO3apS253 phosphopeptide (black stick). (B) 2Fo-Fc map contour at the 1 σ level around the FOXO3apS253 peptide residues (orange mesh), from the cocrystal structure. The map clearly shows the FOXO3a peptide region. (C) Phosphate moiety of FOXO3apS253 (black stick) anchored on the conserved Tyr131, Arg130, Arg57, and Lys50 residues of 14-3-3ε (green stick). Salt bridge interactions were observed for FOXO3aArg249 and FOXO3aAsp255 with Glu183 and Lys50 of 14-3-3ε, respectively. (D) 14-3-3ε residues creating a hydrophobic environment for FOXO3aV252 and FOXO3aM254. The unique intramolecular interaction of FOXO3aR249 with FOXO3apS253 is shown.