TABLE 1.
Expt | Description |
---|---|
2-min CAM | Exposure of oocysts to dissolved CAM in acetone for 2 min, 4-min centrifugation, rinse in 1 ml of acetone, centrifugation; rinse in 1 ml of 95% ethanol, centrifugation; rinse in 1 ml of 70% ethanol, centrifugation; rinse in 1 ml of elution buffer, centrifugation ; wash in bleach |
1-min CAM | Exposure of oocysts to dissolved CAM in acetone for 1 min; 4-min centrifugation; rinse in 1 ml of acetone, centrifugation; rinse in 1 ml of 95% ethanol, centrifugation; rinse in 1 ml of 70% ethanol, centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach |
CAM-acetone | Exposure of oocysts to dissolved CAM in acetone for 2 min; 4-min centrifugation; rinse in 1 ml of acetone, centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach |
CAM only | Exposure of oocysts to dissolved CAM in acetone for 2 min; 4-min centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach |
Acetone only | Oocysts washed in 1 ml of acetone; 4-min centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach |
Ethanol only | Oocysts washed in 1 ml of 95% ethanol; 4-min centrifugation; oocysts washed in 1 ml of 70% ethanol, centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach |
Elution buffer only | Oocysts washed in 1 ml of elution buffer; 4-min centrifugation; wash in bleach; control oocysts washed in PBS before bleach treatment |
Following bleach treatment, the oocysts were rinsed in elution buffer, centrifuged for 4 min at 11,750 × g, and resuspended in 1 ml of growth medium. For each experiment, complementary control samples were used at the same time and from the same oocyst lot.