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. 2001 Jul;67(7):3309–3313. doi: 10.1128/AEM.67.7.3309-3313.2001

TABLE 1.

Experiments designed to test the effects of the CAM dissolution protocol on C. parvum infectivitya

Expt Description
2-min CAM Exposure of oocysts to dissolved CAM in acetone for 2 min, 4-min centrifugation, rinse in 1 ml of acetone, centrifugation; rinse in 1 ml of 95% ethanol, centrifugation; rinse in 1 ml of 70% ethanol, centrifugation; rinse in 1 ml of elution buffer, centrifugation ; wash in bleach
1-min CAM Exposure of oocysts to dissolved CAM in acetone for 1 min; 4-min centrifugation; rinse in 1 ml of acetone, centrifugation; rinse in 1 ml of 95% ethanol, centrifugation; rinse in 1 ml of 70% ethanol, centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach
CAM-acetone Exposure of oocysts to dissolved CAM in acetone for 2 min; 4-min centrifugation; rinse in 1 ml of acetone, centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach
CAM only Exposure of oocysts to dissolved CAM in acetone for 2 min; 4-min centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach
Acetone only Oocysts washed in 1 ml of acetone; 4-min centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach
Ethanol only Oocysts washed in 1 ml of 95% ethanol; 4-min centrifugation; oocysts washed in 1 ml of 70% ethanol, centrifugation; rinse in 1 ml of elution buffer, centrifugation; wash in bleach
Elution buffer only Oocysts washed in 1 ml of elution buffer; 4-min centrifugation; wash in bleach; control oocysts washed in PBS before bleach treatment
a

Following bleach treatment, the oocysts were rinsed in elution buffer, centrifuged for 4 min at 11,750 × g, and resuspended in 1 ml of growth medium. For each experiment, complementary control samples were used at the same time and from the same oocyst lot.