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. 2022 May 23;25(6):104443. doi: 10.1016/j.isci.2022.104443

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Isolation of TgPKS2 AT and AT-E constructs

(A) Overlaid homology models of 3 TgAT domains from TgPKS2, highlighting the N-terminal extensions present in AT-E constructs.

(B) Depiction of each AT and AT-E domain construct and analysis of purified proteins by SDS-PAGE stained with Coomassie blue. Lane 1: BenchMark protein ladder, lane 2: AT1 (35.8 kDa), Lane 3: AT1-E (41.4 kDa), Lane 4: BenchMark protein ladder, lane 5: AT2 (34.8 kDa), Lane 6: AT2-E (40.5 kDa), Lane 7: BenchMark protein ladder, lane 8: AT3-E (41.5 kDa), Lane 9: AT3 (35.7 kDa).