REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-mouse PD-1 (CD279) (J43)	BioXcell	Cat# BE0033-2, RRID:AB_1107747
polyclonal Armenian hamster IgG antibody	BioXcell	Cat# BE0091, RRID:AB_1107773
anti-mouse CD3ε (145-2C11)	BioXcell	Cat# BE0001-1, RRID:AB_1107634
anti-mouse CD28 (37.51)	BioXcell	Cat# BE0015-1, RRID:AB_1107624
anti-human CD3e (OKT3)	BioXcell	Cat# BE0001-2, RRID:AB_1107632
anti-human/monkey CD28 (CD28.2)	BioXcell	Cat# BE0291, RRID:AB_2687814
iTAg Tetramer/PE – H-2 Kb OVA (SIINFEKL)	MBL int corporation	Code: TB-5001-1
CD8 MicroBeads, human	Miltenyi Biotec	Order no 130-045-201
CD8a (Ly-2) MicroBeads, mouse	Miltenyi Biotec	Order no 130-117-044
anti-human CD3 Antibody (OKT3)	eBioscience	Cat# 48-0037-42; RRID:AB_1272055
anti-human CD8a Antibody (RPA-T8)	eBioscience	Cat# 12-0088-42; RRID:AB_1724104
anti-human CD25 Antibody (CD25-4E3)	eBioscience	Cat# 17-0257-42; RRID:AB_11218671
anti-human CD279 (PD-1) Antibody (MIH4)	eBioscience	Cat# 11-9969-42; RRID:AB_10548937
anti-mouse CD3e Antibody (145-2C11)	eBioscience	Cat# 48-0031-82; RRID:AB_10735092
anti-mouse CD8a Antibody (53-6.7)	eBioscience	Cat# 25-0081-82; RRID:AB_469584
anti-mouse CD279 (PD-1) Antibody (J43)	eBioscience	Cat# 11-9985-82; RRID:AB_465472
anti-mouse CD25 Antibody (PC61.5)	eBioscience	Cat# 45-0251-82; RRID:AB_914324
anti-mouse/human Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody (n3kobu5)	eBioscience	Cat# 17-9006-42; RRID:AB_2573268
anti-mouse/human IRAP (D7C5) XP Rabbit mAb antibody	Cell Signaling Technology	Cat# 6918; RRID:AB_10860248
anti-mouse/human Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb antibody	Cell Signaling Technology	Cat# 73382; RRID:AB_2799838
anti-human CD45 (HI30) 100Tst antibody	BD Biosciences	Cat# 612891; RRID:AB_2870179
anti-human CD3e (HIT3a) antibody	BD Biosciences	Cat# 740283; RRID:AB_2740022
anti-human CD4 (M-T477) antibody	BD Biosciences	Cat# 750175; RRID:AB_2874380
anti-human CD8 (HIT8A) 25Tst antibody	BD Biosciences	Cat# 566856; RRID:AB_2869910
anti-human CD279 (MIH4) antibody	BD Biosciences	Cat# 564323; RRID:AB_2738745
anti-mouse Neuropilin-1 Affinity Purified PAb antibody	R and D Systems	Cat# FAB566A; RRID:AB_2267476
anti-human Neuropilin-1 MAb (Cl 446921) antibody	R and D Systems	Cat# FAB3870A; RRID:AB_1241850
donkey anti-rat IgG (H + L) Highly Cross-Adsorbed Secondary Antibody	Thermo Fisher Scientific	Cat# A48269TR; RRID:AB_2896335
goat anti-hamster IgG (H + L) Cross-Adsorbed Secondary Antibody	Thermo Fisher Scientific	Cat# A-21451; RRID:AB_2535868
donkey anti-goat IgG (H + L) Cross-Adsorbed Secondary Antibody	Thermo Fisher Scientific	Cat# A-11057; RRID:AB_2534104
duolink in situ PLA probe anti-rabbit MINUS antibody	Sigma-Aldrich	Cat# DUO92005; RRID:AB_2810942
duolink in situ PLA probe anti-mouse PLUS antibody	Sigma-Aldrich	Cat# DUO92001, RRID:AB_2810939
duolink in situ PLA probe anti-goat PLUS antibody	Sigma-Aldrich	Cat# DUO92003
polyclonal rabbit anti-human NRP1 antibody (Asp525)	LifeSpan Biosciences	Cat# LS-C177530
anti-mouse NRP1 [EPR3113] antibody	Abcam	Cat# ab81321; RRID:AB_1640739
anti-mouse PD-1 (ab1) antibody	Sigma-Aldrich	Cat# PRS4065; RRID:AB_1855098
anti-mouse PD-1 antibody	Abcam	Cat# ab117420; RRID:AB_10902420
anti-mouse Neuropilin-1 Affinity Purified PAb antibody	R and D Systems	Cat# AF566; RRID:AB_355445
anti-CD8b (H35–17.2) unconjugated monoclonal antibody	BD Biosciences	Cat# 550797; RRID:AB_393886
Bacterial and virus strains
rAAV1/SPc5-12-mOVA	Bartolo et al. (2019)	N/A
Chemicals, peptides, and recombinant proteins
Albumin from chicken egg white	Sigma-Aldrich	CAS number: 9006-59-1
Poly(I:C) HMW	Invivogen	CAS number: 31852-29-6
Staphylococcal enterotoxin B from Staphylococcus aureus (SEB)	Sigma-Aldrich	CAS number: 11100-45-1
Zombie Aqua Fixable Viability dye	Biolegend	Cat No 423101
CellTrace Violet	Thermo fisher	Cat No C34557
Fixable Viability Dye eFluor 450	Thermo fisher	Cat No 65-0863-14
Phalloidin AF555	Thermo fisher	Cat No A34055
Deposited data - Data accession numbers
Rossignol, Julien (2022), “Neuropilin-1 cooperates with PD-1 in CD8+ T-cells predicting outcomes in melanoma patients treated with anti-PD1”,	Mendeley Data, V1	https://doi.org/10.17632/5xgbf6fdty.1
Experimental models: Cell lines
EL4-CFP cell line (EL4 cell line transfected with membrane-bound CFP)	ATCC	Cat# TIB-39, RRID:CVCL_0255
A20 cell line	ATCC	Cat# TIB-208, RRID:CVCL_1940)
B16-OVA cell line (B16-F10 transfected with ovalbumin)	ATCC	Cat# CRL-6475, RRID:CVCL_0159)
TC1 cell line	ATCC	Cat# JHU-1, RRID:CVCL_4699)
Experimental models: Organisms/strains
Mice: B6.129(SJL)-Nrp1tm2Ddg/J	Jackson Laboratory, California, USA	Cat# JAX:005247; RRID:IMSR_JAX:005247
Mice: C57BL/6-Tg 1Itan/J	Jackson Laboratory, California, USA	Cat# JAX:008766; RRID:IMSR_JAX:008766
Mice: C57BL/6J	Janvier Labs, France	Cat# 5752053; RRID:MGI:5656904
Mice: B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)1100Mjb	Gift from Jean Davoust, INSERM U1151	catalog # TAC:2334; RRID:IMSR_TAC:2334
Experimental models and subject details
Patient with NRP1 haploinsufficiency	Gender: Male	Age: 16
Healthy donors	Gender: Female 3 Male 2	Median age: 22 [18:32]
Melanoma patients	Gender: Female 15 Male 13	Median age: 69 [24;89]
Software and algorithms
GraphPadPrism, version 5.0	GraphPad Software	GraphPad Prism; RRID:SCR_002798
FlowJo v10 software	Tresstar Inc	FlowJo; RRID:SCR_008520
INSPIRE software	Amnis Corp, Millipore	RRID:SCR_018589
IDEAS software	Amnis Corp, Millipore	RRID:SCR_018589