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. 2022 Jun 2;81:104090. doi: 10.1016/j.ebiom.2022.104090

Figure 2.

Fig 2

Magnitude and polyfunctionality of HIV-specific CD8+T cell responses in PLWH on ART. N=35 PLWH (a) Spearman correlation of fold-change in IFNγ expression from total live CD8+ T cells after Gag-peptide presentation by MDDCs, highlighting optimal response established as a minimum of 2.5 fold-change in IFNγ from baseline. Spearman r and p values are shown on the left for each correlation. (b) Left panel showing ROC curve for classification of our cohort based on CD8+ T cell IFNγ response to Gag-loaded MDDC and years under treatment of each individual; and right panel showing pie-charts of the stratification of PLWH based on less than 10 years (ST-ARTp, light grey), or equal or more than 10 years (LT-ARTp, dark grey) of ART duration. ROC curve was calculated with SPSS v20 and statistical significances of pie charts was calculated using a Chi-square test with Yates’ correction (***p<0.001). (c-d) Fold-change in IFNγ expression (c) and fold-change in polyfunctional responses (CD107a+ IFNγ+ cells) (d) from total live CD8+ T cells after stimulation of MDDCs in ST-ARTp (left plots, in light grey) and LT-ARTp (right plots, in dark gray). Statistical significance was calculated using two-tailed Wilcoxon test (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001).