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. 2022 Jun 2;81:104090. doi: 10.1016/j.ebiom.2022.104090

Figure 5.

Fig 5

CD8+T cell oxidative and glycolytic metabolism in PLWH. (a-b) Oxygen consumption rate (a; OCR) and extracellular acidification rate (b; ECAR) in CD8+ T cells from PLWH comparing basal (light line) and TCR activated (dark line) cells from ST-ARTp (N=10; upper plots; light grey), LT-ARTp (N=10; middle plots; dark grey), and HIV-1 negative controls (HC; N=12; lower plots; green). Statistical significance per each timepoint was calculated with a multiple t test analysis. (c) RT-qPCR analysis of HIF1α (upper) and GLUT1 (lower) transcriptional expression normalized to β−actin mRNA levels in memory CD45RA− CD8+ T cells isolated from total PBMCs from HIV negative controls (HC; N=9; green) and PLWH (ST-ARTp; N=9; light grey, and LT-ARTp; N=9; dark grey). Statistical significance between double and single positive populations was calculated using two-tailed Wilcoxon test (*p<0.05).