Fig. 1.

Effects of GX_P2V infection on the aberrant glycosylation of proteins in Vero E6 cells. A. Effects of GX_P2V treatment on glycosylation (sites or proteins) in presence or absence of CEP. Fold change (Vero_C_P/Vero or Vero_P/Vero) ≥2 or ≤0.5 was considered as up-regulation or down-regulation, respectively. B. Global presentation of proteomic and respective glycosylation occupancy based on the effects of GX_P2V infection. Yellow dots represent proteins which have not changed significantly in global proteomics data but the peptides with glycosylation have differentially expressed over 2 folds, and blue dots represent proteins which are differentially expressed both in the levels of global protein and glycosylation. C. Protein-protein interaction (PPI) network and the interaction modules of key proteins in response to GX_P2V treatment. The PPI network of 66 differentially expressed glycoproteins (Fc ​≥ ​2 or Fc ​≤ ​0.5) in GX_P2V treatment was produced using STRING and Cytoscape. D. The key interaction module was obtained from the PPI network by Cytoscape with MCODE plugin using differentially expressed glycosproteins in GX_P2V-treated Vero E6 cells. Proteins marked in red color represent those proteins containing up-regulated glycosylation sites, the blue ones indicate proteins containing down-regulated glycosylation sites, and the purple color labelled protein contains both up- and down-regulated glycosylation sites. E. Functional enrichment analysis of the key modules induced by GX_P2V treatment was obtained from Metascape. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)