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. 2022 Jul 7;13:897873. doi: 10.3389/fimmu.2022.897873

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Copyright © 2022 Rudolph, Wang, Simolka, Huc-Claustre, Dai, Grotenbreg, Besra, Shevchenko, Shevchenko and Zeissig

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic overview of cloning and purification of SortA-CD1d. N-ter/C-ter: N-terminal and C-terminal end of peptides and proteins; α1-3: alpha helical domain 1-3 of CD1d; TM, transmembrane domain; CD, cytosolic domain; LPETG, five amino acid long recognition motif of Sortase A; GGG-AF, triglycine labeled affinity tag.