Figure 2.

SortA-CD1d but not SortA-TD-CD1d traffics through the endolysosomal system. L cells stably transfected with WT-CD1d, SortA-CD1d or SortA-TD-CD1d were stained with antibodies against the lysosomal marker LAMP1 (A, red) or the early endosomal marker EEA1 (red, B). Cells were stained with antibodies against CD1d(green, A, B) and nuclei were counterstained with DAPI (blue). Scale bars represent 5 μm. Figures are representative of 2 independent experiments.