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. 2022 Jul 7;13:897873. doi: 10.3389/fimmu.2022.897873

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Copyright © 2022 Rudolph, Wang, Simolka, Huc-Claustre, Dai, Grotenbreg, Besra, Shevchenko, Shevchenko and Zeissig

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SortA-CD1d and SortA-TD-CD1d can load lipid antigens and present them to NKT cells. L cells stably transfected with SortA-CD1d or SortA-TD-CD1d were loaded with (A) αGalCer, (B) αGalGalCer or (C) C20:2 and were subsequently co-cultured with NKT hybridoma cells. IL-2 concentration as a measure of NKT cell activation was determined by ELISA in cell culture supernatants. Graphs show mean ± SD of biological duplicates and are representative for one out of at least two independent experiments.