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. 2022 Jul 7;13:897873. doi: 10.3389/fimmu.2022.897873

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Copyright © 2022 Rudolph, Wang, Simolka, Huc-Claustre, Dai, Grotenbreg, Besra, Shevchenko, Shevchenko and Zeissig

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sortase A allows for efficient cleavage, tagging, and purification of H-2Kb, CD1d, and CD1d-TD. (A) ELISA-based analysis of β2-microglobulin in different purification fractions. FT: flow through, W1-6: wash fractions, E1-5: elution fractions. (B) Coomassie blue-stained SDS gel of elution fraction 3 (E3) of SortA-H-2Kb, SortA-CD1d, and SortA-TD-CD1d. 1: mouse heat shock protein 70, 2a: mCD1d, 2b: H-2Kb 3: Sortase A, 4: β2m and Strep-Tactin^®. M: PageRuler™ unstained protein ladder. Results are representative of 4 independent experiments.