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. 2022 Jul 7;13:897873. doi: 10.3389/fimmu.2022.897873

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Copyright © 2022 Rudolph, Wang, Simolka, Huc-Claustre, Dai, Grotenbreg, Besra, Shevchenko, Shevchenko and Zeissig

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipids associated with SortA-CD1d and SortA-CD1d-TD. SortA-CD1d, SortA-TD-CD1d, and SortA-H-2Kb were purified as described in Methods and subjected to shotgun lipidomics. Only lipids identified in 3 of 4 independent experiments and showing (A) at least three -fold higher or (B) at least two-fold higher abundance in SortA-CD1d- or SortA-TD-CD1d purifications compared to SortA-H-2Kb purifications are shown. Lipid abundance is expressed as pmol lipid per pmol of the respective protein with error bars indicating mean ± SEM. Statistics: Kruskal-Wallis followed by Dunn´s post-hoc test, p-value * < 0.05; Asterisks indicate significance in the Dunn´s post-hoc test of a comparison of the group indicated by asterisk (SortA-CD1d or SortA-TD-CD1d) compared to SortA-H-2Kb.