TABLE 1.
Purification step | Protein (mg) | Activity (U) | Sp act (U/mg) | Purification (fold) | Yield (%) |
---|---|---|---|---|---|
Culture filtrate | 473.0 | 228.0 | 0.48 | 1 | 100 |
(NH4)2SO4 | 199.0 | 211.0 | 1.06 | 2.2 | 92.5 |
DEAE-Toyopearl | 31.7 | 18.9 | 0.60 | 1.3 | 8.3 |
Phenyl Superose | 2.6 | 5.2 | 2.00 | 4.2 | 2.3 |
Mono Q | 0.6 | 2.5 | 4.17 | 8.7 | 1.1 |