Extended Data Fig. 8. Target cleavage by EfPiwi and Siwi (part I).

a, Denaturing gels showing cleavage of g2-g21 matched ³²P-labeled target RNA by EfPiwi, hAgo2 or Siwi in the presence of various divalent cations (2 mM each). Schematic of piRNA-target pairing shown (top). Gels are representative results for n=3 independent experiments for EfPiwi and hAgo2, and n=2 independent experiments for Siwi. b, Time course showing cleavage of g2-g21 paired ³²P-labeled target RNA by EfPiwi in the presence of Mg²⁺, Mn²⁺, or both at approximate physiological divalent cation concentrations. c, Cleavage of g2-g21 matched ³²P-labeled target RNA by EfPiwi at various temperatures shows activity over the full physiological range (17–30°C). Gel is representative of n=3 independent experiments. d, Quantitation of results (and replicates) in c. e, Cleavage of target RNAs with varying degrees of 3’ complementarity by EfPiwi at 30°C. In b, d and e, n=3 independent experiments, data are mean ± s.d.