a, Schematic of guide RNAs used. b, Fraction of target RNAs bound by hAgo2-guide, EfPiwi-guide, and Siwi-guide RNA complexes at equilibrium versus protein-guide concentration. c, Release rates of g2-g8 complementary target protein-guide RNA complexes. d, Release of 32P-labeled target RNAs from indicated protein-guide complexes in the presence of excess unlabeled target RNA over time. In b-c
n=3 independent experiments, data are mean ± s.d.