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GmhB is required for normal LPS composition. LPS from 1 × 109 CFU of KPPR1, ΔgmhB, ΔgmhB+pACYCgmhB, or ΔgalU was isolated and 10 μL of yield was analyzed by polyacrylamide electrophoresis. LPS core regions in interest are labeled in a, b, and c. The gel displayed is representative of three independent trials, duplicate lanes represent independent LPS preparations. The CandyCane glycoprotein molecular weight standard is displayed in the left lane.
