TABLE 1.
Antibody panels
| Marker | Colorb | Manufacturer, catalog no.a |
|---|---|---|
| Panel 1 | ||
| CCR2 | BV786 | BD, 747966 |
| CD11b | BV510 | BD, 562950 |
| CD11c | PerCP/Cy5.5 | BL, 117328 |
| CD207 | APC | MB, 130-113-053 |
| F4/80 | BV650 | BD, 744338 |
| Ly6C | AF700 | BL, 128023 |
| Ly6G | FITC | BD, 551460 |
| MHC-II (I-Ab) | PE | BD, 553552 |
| Dead cells | DAPI | BD, 561907, or Invitrogen, D1306 |
| Panel 2 | ||
| CD11b | BV510 | BD, 562950 |
| CD11c | PerCP/Cy5.5 | BL, 117328 |
| CD45 | BV711 | BD, 563709 |
| CD3ε | BV605 | BL, 100351 |
| CD90.2 | APC | BD, 561974 |
| GR1 | FITC | BD, 553126 |
| NK1.1 | PE | BD, 553165 |
| B220 | BV786 | BD, 563894 |
| Dead cells | DAPI | BD, 561907, or Invitrogen, D1306 |
| Panel 3 | ||
| CD45 | PE-CF594 | BD, 562420 |
| CD3ε | BV605 | BL, 100351 |
| CD4 | PE-Cy5 | BL, 130312 |
| CD8α | BV711 | BL, 100759 |
| NK1.1 | APC | MB, 130-120-507 |
| γδTCR | FITC | eB, 11-5711-82 |
| Dead cells | DAPI | BD, 561907, or Invitrogen, D1306 |
a
BD, BD Biosciences; BL, BioLegend; MB, Miltenyi Biotec; eB, eBioscience.
b
PerCP, peridinin chlorophyll protein; APC, allophycocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin; BV, brilliant violet; AF, alexa fluor.