FIG 1.

Expression of predicted toxin–antitoxin systems under different conditions and Bpm K96243 survival after antibiotic treatment. (A) Heatmap with the predicted toxin and antitoxin genes (left) from Bpm K96243 and the conditions evaluated on the bottom (left group, antibiotics exposition; right group, intracellular survival bacteria at 12 h and 24 h). As control of expression, bacteria were grown in media (LB or DMEM) without supplements. Each condition was measured at least in triplicate from three independent replicates. (B) CFU enumeration and (C) survival based on the input after 24 h of supralethal antibiotic concentration exposure of Bpm K96243 to LEV, levofloxacin; CIP, ciprofloxacin; DOX, doxycycline; CFT, ceftazidime; MER, meropenem; Control, LB medium.