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Overexpression of toxin induced system in nutrient rich or minimal media and killing curves in the presence of quinolones. Growth curves of empty pBAD vector and the one carrying the toxin BPSL3343 (predicted HigB) after induced with arabinose in (A) LB or (B) minimal M9 media. Killing curves of Bpm K96243, ΔhigB, and ΔhigB ΔhigA in presence or supralethal concentrations of (C) levofloxacin or (D) ciprofloxacin. *, P < 0.05; **, P < 0.01.
