FIG 6.

Uptake and survival under macrophage induced-persistence conditions. Murine macrophages were infected (MOI 10) with the different Bpm strains for 30 min. The uptake percentage was calculated from the input bacteria (A). After 30 min, media were removed and replaced with fresh media supplemented with kanamycin for (B) 3 h, (C) 6 h, or (D) 12 h, or with the cell permeable levofloxacin for (E) 12 h and (F) 24 h. Bars represent the average of three independent experiments in triplicate ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.