TABLE 3.
List of primers designed and used in this study
| Primer | Sequence | Experiment |
|---|---|---|
| F1F_3343 | GAGCTGATATCAGGGCCCCGTAATTCTTCTTGCTTCTACTCCATC | Mutant construction |
| F1R_3343 | TGCGGACTACAGAGGTGCGTAATGGCTAAG | |
| F2F_3343 | ACGCACCTCTGTAGTCCGCAAGAATCCCAG | |
| F2R_3343 | GATTAATTGTCAACAGCTCATGAATAGTGCGGCGCTGTC | |
| F1F_RS | GAGCTGATATCAGGGCCCCGATCTCGATCCCATCCGGC | |
| F1R_RS | TCTGCTCGCATTGTGATTTCGTTCGCTTGAC | |
| F2F_RS | GAAATCACAATGCGAGCAGACGAGCTGTG | |
| F2R_RS | GATTAATTGTCAACAGCTCAAAATTTGCTAACATGAAATTCGATTGGACG | |
| BPSL3343-BPS_RS18025 F | CCGCCTTGACCTTACTATCTG | Coexpression |
| BPSL3343-BPS_RS18025 R | GTTTGAGTTGCGTCCAAAGG | |
| BPSL3343 F | CTGGGATTCTTGCGGACTAC | qPCR |
| BPSL3343 R | GCAACTCAAACAACCCGTTAC | |
| BPS_RS18025 F | GCTCGCAGATAGTAAGGTCAAG | |
| BPS_RS18025 R | CTTGAGTCAATCCAGCCTCTT | |
| BPSS0390 F | GATGGCTGGAGGTTGGTT | |
| BPSS0390 R | CCAATCGGTAGGTCCTTCTTC | |
| BPSS0391 F | TGATCGAGCTTGGAGAAGATG | |
| BPSS0391 R | GCTTGGAATCAAGCTGAGAAAG | |
| BPSS0394 F | GACATTGTCGACGCCTTCA | |
| BPSS0394 R | CTACGCAGGCTGATGTTCC | |
| BPSS0395 F | CCTCTATTGCGTGGTGTTCA | |
| BPSS0395 R | GCTTGCTCGACATAGCTCTT | |
| BPSL0175 F | TCGGATCGAACTCATCATGC | |
| BPSL0175 R | CCTTCCATGTCGACACCTG | |
| BPSL0174 F | GAATGTCACCGCGAGTCTT | |
| BPSL0174 R | CTGGAGTCGATGTGTGATCTG | |
| BPSL0559 F | CCTGAGCACCGACTTTCAATA | |
| BPSL0559 R | CAGCCATCAGGCTCAGTATC | |
| BPSL0558 F | GGGCTTTCTACGTCATCCAT | |
| BPSL0558 R | GGAGGAGATTCAGGATGTTCAG | |
| BPSL2333 F | TTAGCCCTGGTGCAAATGA | |
| BPSL2333 R | TTGTGATGCACCACCATCT | |
| BPSL2334 F | ACCGGATGAGGTCGAGAT | |
| BPSL2334 R | CGCGCAACCATTTGTCATAG | |
| BPSL3261 F | CCTCTTTGTGGTCGTCTTCTATC | |
| BPSL3261 R | ACTTCAACTGTTCCGCATACT | |
| BPSL3260 F | GACCTTGCAGAGCAACTATGA | |
| BPSL3260 R | ATCCACCTCACCATGCAAAT | |
| BPSS1060 F | CATGGACGAGATACCGAGATG | |
| BPSS1060 R | GAAACGTGAGGAATACGCAATAG | |
| BPSS1061 F | TTCGACGGTGTTTCTGATCG | |
| BPSS1061 R | GCGGCACACGTCGTATT | |
| BPSS1583 F | AACGGCAAGACCGACAA | |
| BPSS1583 R | CATACGGCTTGATGAAGTTGAC | |
| BPSS1584 F | CGTCGAGGAACGCATCAC | |
| BPSS1584 R | TTGTCGCGCATCGTATCG | |
| BPSL0033 F | CGAGGTCGCCTCGATGTT | |
| BPSL0033 R | GAGGTCAGCCTGCGGAT | |
| BPSL0034 F | GCGAGCTTCGATACGATCATT | |
| BPSL0034 R | CGTCATGAACGGGTTCTCC | |
| BPSL0952 F | TCGCCTTGACAAGCATACAG | |
| BPSL0952 R | GTCCATGTTCAGCCTCAAGTAG | |
| BPSL0953 F | TGCCCGAAGTGCTCAAA | |
| BPSL0953 R | TCAGGCGGTCAATGTCAC | |
| 16S F | GCGTAGAGATGTGGAGGAATAC | |
| 16S R | ACCAGGGTATCTAATCCTGTTTG | |
| rpoB F | CCGAAGGACGTGCTGTATTT | |
| rpoB R | GTGAAGTTGTCGAAGACGAAGA |