FIG 8.
Promotion of keratin gene expression associated with epithelial cell proliferation in the Keap1FA/–::Nrf2SA/SA esophageal epithelium. (A) k-means clustering of gene expression levels in esophagus samples from Keap1+/+::Nrf2+/+, Keap1+/+::Nrf2SA/SA, Keap1FA/FA::Nrf2+/+, Keap1FA/FA::Nrf2SA/SA, Keap1FA/–::Nrf2+/+, and Keap1FA/–::Nrf2SA/SA mice; (B) enriched pathways in GO Biological Process for clusters A and D. (C) Enriched pathways in ENCODE and ChEA consensus TFs from ChIP-X for clusters A and D. (D) mRNA levels of Nrf2 target genes related to the stress response, metabolic enzymes, and keratin by qRT-PCR. Hprt was used as the internal control. The mRNA levels in the Keap1+/+::Nrf2+/+ mice were set to 1. The bar graphs show the means ± SD. **, P < 0.01 by one-way ANOVA with Tukey’s multiple-comparison test.