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. Author manuscript; available in PMC: 2022 Jul 21.
Published in final edited form as: Regen Med. 2014 Jan;9(1):41–51. doi: 10.2217/rme.13.76

Figure 3. Reactivation of the tethered BMP-2 in transglutaminase-crosslinked gelatin gel by NIH 3T3 cells.

Figure 3.

(A) Gelatin zymograph showed native MMP-2 and MMP-9 secretion from NIH 3T3 cells for days 1–5. MMP-2 and MMP-9 have the molecular weight of 72 and 92 kDa, respectively. (B) ALP activity was tested by harvesting the cell culture medium of NIH 3T3 that was incubated with BMP-2–Tg–Gel on days 1–5. C2C12 cells were exposed to each medium for 48 h at 37°C before the ALP activity assay (n = 4). Data are expressed as mean ± standard deviation.

*p < 0.05; **p < 0.01.

BMP-2–Tg–Gel: Transglutaminase-crosslinked gelatin gel with BMP-2; Tg–Gel: Transglutaminase-crosslinked gelatin gel.